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adaptor  (New England Biolabs)


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    Structured Review

    New England Biolabs adaptor
    Adaptor, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 33541 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/adaptor/T4+DNA+Ligase/pmc12996642-80-28-36
    Average 99 stars, based on 33541 article reviews
    adaptor - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    other:

    Article Title: Sphingomonas paucimobilis-Driven Epithelial-Endothelial Transition in Adenomyosis Pathogenesis.
    Article Snippet: Approximately 1–200 ng of DNA was digested with 4 U of BcgI (NEB) for 3 h at 37◦C.

    Article Title: The discovery of 5mC-selective deaminases and their application to ultra-sensitive direct sequencing of methylated sites at base resolution.
    Article Snippet: The isolated plasmid DNA was hydrolyzed to nucleosides using the Nucleoside Digestion Mix (NEB #M0649S) performed at 37 ◦ C for >2 hours.

    Ligation:

    Article Title: Co-option of a mouse-specific retrotransposon rewires Ash2l isoform usage to prime developmental promoters
    Article Snippet: RNA was fragmented, and 3’ ends were fixed by QuickCIP treatment (NEB, 30 U). .. Pre-adenylated 3’ adaptor (2 μM) ligation was carried out by T4 RNA ligase 2, truncated KQ (NEB, 200 U). .. Reverse transcription was carried out by SuperScript III (2 μl, Invitrogen).

    Article Title: Longitudinal Characterization of Gingival Microbiota Following Periodontally Accelerated Osteogenic Orthodontics Surgery in Skeletal Class III Orthognathic Patients
    Article Snippet: DNA (1 pg-200 ng) was digested with 4 U of BcgI (NEB) for 3 hours at 37 °C. .. Adaptors were then ligated to the DNA fragments by mixing 5 μl of digested DNA with 10 μl of a ligation master mix containing 0.2 μM of each adaptor and 800 U of T4 DNA ligase (NEB). ..

    Article Title: A versatile CRISPR-associated transposases-mediated toolkit for transcriptional regulation library
    Article Snippet: .. MmeI-digested gDNA was ligated to a double-stranded i5 universal adaptor containing a 3′-terminal NN overhang ( ) in a 20 μl ligation reaction containing 16.33 μl of MmeI-digested gDNA, 500 nM adaptor, 400 U T4 DNA ligase (NEB), and 1 × T4 DNA ligase buffer. ..

    Article Title: Intratumoral immune-microbial crosstalk shaped by tumor cell-derived extracellular vesicles encapsulating berberine boosts lung cancer immunotherapy
    Article Snippet: .. This ligation process involved mixing 5 μL of digested DNA with 10 μL of a master mix containing 0.2 μmol/L of each adaptor and 800 units of T4 DNA ligase (NEB), incubated at 4 °C for 12 h. Following ligation, the products were amplified, and the resulting PCR products were separated on an 8% polyacrylamide gel. ..

    Article Title: Longitudinal Characterization of Gingival Microbiota Following Periodontally Accelerated Osteogenic Orthodontics Surgery in Skeletal Class III Orthognathic Patients.
    Article Snippet: DNA (1 pg-200 ng) was digested with 4 U of BcgI (NEB) for 3 hours at 37 °C. .. BI PD KEG (mm) (mm) w 0 2w 4w 0 2w 4w 0 2w 4w 2 1 1 3 3.5 3 4.0 4.5 4.7 1 1 1 2 2 2 5.3 5.7 5.9 1 2 1 2 3 2 4.5 5.1 5.3 1 1 1 2 2 3 6.0 6.4 6.5 1 2 1 2 2 2 4.8 5.3 5.7 1 2 2 2 3 2 5.4 5.8 6.0 inized gingiva width (mm); PD, probing depth (mm); PLI, plaque index; Adaptors were then ligated to the DNA fragments by mixing 5 ml of digested DNA with 10 ml of a ligation master mix containing 0.2 mM of each adaptor and 800 U of T4 DNA ligase (NEB). .. Genomic DNA was extracted from each gingival crevicular fluid sample using the TIANamp Micro DNA Kit (Tiangen Biotech, Cat# DP316, Beijing, China) according to the manufacturer’s instructions, which are suitable for trace and lowbiomass samples.

    Incubation:

    Article Title: Intratumoral immune-microbial crosstalk shaped by tumor cell-derived extracellular vesicles encapsulating berberine boosts lung cancer immunotherapy
    Article Snippet: .. This ligation process involved mixing 5 μL of digested DNA with 10 μL of a master mix containing 0.2 μmol/L of each adaptor and 800 units of T4 DNA ligase (NEB), incubated at 4 °C for 12 h. Following ligation, the products were amplified, and the resulting PCR products were separated on an 8% polyacrylamide gel. ..

    Amplification:

    Article Title: Intratumoral immune-microbial crosstalk shaped by tumor cell-derived extracellular vesicles encapsulating berberine boosts lung cancer immunotherapy
    Article Snippet: .. This ligation process involved mixing 5 μL of digested DNA with 10 μL of a master mix containing 0.2 μmol/L of each adaptor and 800 units of T4 DNA ligase (NEB), incubated at 4 °C for 12 h. Following ligation, the products were amplified, and the resulting PCR products were separated on an 8% polyacrylamide gel. ..

    Polymerase Chain Reaction:

    Article Title: Intratumoral immune-microbial crosstalk shaped by tumor cell-derived extracellular vesicles encapsulating berberine boosts lung cancer immunotherapy
    Article Snippet: .. This ligation process involved mixing 5 μL of digested DNA with 10 μL of a master mix containing 0.2 μmol/L of each adaptor and 800 units of T4 DNA ligase (NEB), incubated at 4 °C for 12 h. Following ligation, the products were amplified, and the resulting PCR products were separated on an 8% polyacrylamide gel. ..



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